![]() Male adrenalectomized rats were challenged with increasing doses of the GR agonist corticosterone (CORT) ranging from 3 to 3000 mu g/kg, resulting in clear differences in the GR-binding profile to individual GBS. Gene ontology clusters involved in neuronal plasticity and other essential neuronal processes were over-represented among the genes harboring a GBS or located in the vicinity of a GBS. Analysis of the genomic distribution of the significant GBS revealed a high prevalence of intragenic GBS. We identified 2470 significant GR-binding sites (GBS) and were able to confirm GR binding to a random selection of these GBS covering a wide range of P values. Show more In the present study, genomic binding sites of glucocorticoid receptors (GR) were identified in vivo in the rat hippocampus applying chromatin immunoprecipitation followed by next-generation sequencing. Two groups of GBS could be distinguished: a low-CORT group that displayed GR binding across the full range of CORT concentrations. ![]() Two Populations of Glucocorticoid Receptor-Binding Sites in the Male Rat Hippocampal Genome Article / Letter to editor In the present study, genomic binding sites of glucocorticoid receptors (GR) were identified in vivo in the rat hippocampus applying chromatin immunoprecipitation followed by next-generation sequencing.
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